EMI1 (EMI1/1176), 0.2mg/mL

• Description:

  This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1) . It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Early Mitotic Inhibitor-1 (EMI1); FBX5; Fbxo31

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FBXO5

• Gene ID:

  26271

• NCBI Gene ID:

  520506

• UniProt:

  Q9UKT4

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

• Target Antigen:

  EMI1

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  EMI1/1176

• Conjugation:

  Purified, with BSA

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  IHC, FFPE (verified) |WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cell cycle

• Positive Control:

  HeLa, 293 or HepG2 cells. Ovarian carcinoma.

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  56 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Western blotting 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA