Anti-P-Cadherin (CDH3) (6A9), CF405S conjugate

Anti-P-Cadherin (CDH3) (6A9), CF405S conjugate

• Description: This antibody recognizes a protein of 116 kDa, identified as P-Cadherin-1 (CDH3). It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cadherin 3 type 1; Cadherin-3; Cadp; Calcium dependent adhesion protein placental; CDH3; CDHP; HJMD; P-cadherin (Placental); PCAD; Placental cadherin
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CDH3
• Gene ID: 1001
• NCBI Gene ID: 191842
• UniProt: P22223
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: A431 trypsinized membranes
• Target Antigen: P-Cadherin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 6A9
• Conjugation: CF405S
• Disease: Tumor
• Source: Animal
• Applications: Functional studies (published) | IF (published) | IHC, FFPE (published) | IP (published) | WB (published)
• Validated Applications: IF, IHC, FFPE, IP, WB
• Field of Research: Cancer, Cell adhesion
• Positive Control: A431 or PC-3 cells. Pancreas, Placenta or Prostate
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 116 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Invest Dermatol (1994) 102:870-877. (WB; IP; adhesion blocking) Cancer (1999) 86(7): 1263-1272. (IHC, FFPE) Cell Comm Adhesion (2002) 9(2): 103-115. (IF; WB; IP) Oncogene (2006) 25: 4595-4604. (IF; WB)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years