Anti-PD1 / PDCD1 / CD279(PDCD1/922), CF405S conjugate

Anti-PD1 / PDCD1 / CD279(PDCD1/922), CF405S conjugate

• Description: PD-1 (also known as Programmed Death 1, CD279, and PDCD1) is a negative regulatory T cell surface receptor. PD-1 is a Type I transmembrane protein expressed on the plasma membrane of T-cells. It is a T-cell checkpoint protein, involved in preventing autoimmunity. Because binding of PD-1 by its ligands PD-L1 or PD-L2 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: CD279; hPD-1; hSLE1; PD1; PDCD1; Programmed Cell Death Protein 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PDCD1
• Gene ID: 5133
• NCBI Gene ID: 158297
• UniProt: Q15116
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human PDCD1 protein
• Target Antigen: CD279 | PD1 | PDCD1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: PDCD1/922
• Conjugation: CF405S
• Disease: Tumor
• Source: Animal
• Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IF, IHC, FFPE
• Field of Research: Apoptosis, Cancer, Cancer immunotherapy/immune checkpoint
• Positive Control: TY cells or Tonsil.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 55 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years