Recombinant Mouse HGF (C-6His)

Recombinant Mouse HGF (C-6His)

• Background: HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis.  It can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions .
• Description: Recombinant Mouse Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln33­Leu728 is expressed with a 6His tag at the C-terminus.
• Synonyms: Hepatopoietin-A; Scatter factor; SF; deafness; autosomal recessive 39; DFNB39; F-TCF; hepatocyte growth factor
• CAS Number: 9000-83-3
• UniProt: Q08048
• Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Molecular Weight: 53.5 & 26.9 KDa
• Shipping Conditions: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
• Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.