Granulocyte Marker (BM-1), 0.2mg/mL

• Description:

  Recognizes 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Myeloid specific protein; p183 DNA-binding cytoplasmic protein

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene ID:

  Not Known

• NCBI Gene ID:

  Not Known

• UniProt:

  Not Known

• Cellular Locus:

  Nucleus & cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human peripheral blood mononuclear cells

• Target Antigen:

  Granulocyte Marker

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  BM-1

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |RIA (published) |WB (published)

• Validated Applications:

  IF, IHC, IHC, FFPE, RIA, WB

• Field of Research:

  Immunology

• Positive Control:

  HL60 cells. Bone marrow, lymph node or tonsil

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  183 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|No special pretreatment is required for staining of formalin/paraffin tissues|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Blood (1987) 70(4): 1124-1130. (IHC, FFPE; IHC, frozen; IF; WB; RIA)

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA