Cattle anti-HAV IgG (anti-Hepatitis A antibody IgG) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Anti-HAV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Anti-HAV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Anti-HAV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Anti-HAV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Hepatitis A virus IgG

• Gene Aliases:

  Hepatitis A virus IgG

• Reactivity:

  Cattle

• Field of Research:

  Infection immunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Anti-HAV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Anti-HAV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Anti-HAV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Anti-HAV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.069 ng/mL

• Species:

  Cattle

• CAS Number:

  9000-83-3, 7732-18-5