Human KLK10 (Kallikrein 10) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1

• Gene Aliases:

  NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1

• UniProt:

  O43240

• Reactivity:

  Human

• Field of Research:

  Enzyme & Kinase, Tumor immunity, Reproductive science

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  10000 pg/mL

• Sample Type:

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  156.25 - 10000 pg/mL

• Sensitivity:

  48.8 pg/mL

• Species:

  Human

• CAS Number:

  7732-18-5