Mouse ERb (Estrogen Receptor Beta) ELISA Kit
CAT:
855-ELK4281-01
Size:
48 Tests
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse ERb (Estrogen Receptor Beta) ELISA Kit
- Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ERb in the samples is then determined by comparing the OD of the samples to the standard curve.
- Gene Aliases: ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2
- UniProt: O08537
- Field of Research: Endocrinology; Reproductive science; Hormone metabolism.
- Assay Principle: Sandwich ELISA
- Assay Performance Time: 3.5h
- Standard: 20 ng/mL
- Sample Type: Tissue homogenates, cell lysates and other biological fluids.
- Detection Range: 0.32-20 ng/mL
- Sensitivity: 0.145 ng/mL