Simian CTXI (CrossLinked C-telopeptide of Type I Collagen) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CTXⅠ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CTXⅠ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CTXⅠ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CTXⅠ in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  CTX-I; CTX1; CTXI

• Gene Aliases:

  CTX-I; CTX1; CTXI

• Reactivity:

  Simian

• Field of Research:

  Immunology, Cardiovascular, Signal Transduction, Cancer, Metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CTXI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CTXI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CTXI in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.09 ng/mL

• Species:

  Simian

• CAS Number:

  7732-18-5