CD90 (AF-9), 0.2mg/mL

• Description:

  Recognizes a protein of 18-35 kDa, identified as CD90 (also known as Thy1) . CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34 cells in bone marrow, and Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  THY1

• Gene ID:

  7070

• NCBI Gene ID:

  644697

• UniProt:

  P04216

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Cow, Human

• Immunogen:

  Human T-lymphoma cells

• Target Antigen:

  CD90 | Thy1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  AF-9

• Conjugation:

  Purified, with BSA

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  Flow, surface (published) |IF (published) |IHC, FFPE (published)

• Validated Applications:

  FC, IF, IHC, FFPE

• Field of Research:

  Cell adhesion, Immunology

• Positive Control:

  IMR-32, CCRF-CEM or MOLT-4 cells. Human uterus.

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  18-35 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Int J Stem Cell Res Ther (2018) 5:056. (IHC, FFPE) Biology Open 2017 6: 1423-1433. (Flow) Gastroenterol (2009) 136(2): P705-714. (IF)

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA