CD53 (63-5A3), 0.2mg/mL

• Description :

  Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop V; Code CD53.1) . CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms :

  CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25) ; TSPAN25

• UNSPSC :

  41116161

• UNSPSC Description :

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name :

  CD53

• Gene ID :

  963

• NCBI Gene ID :

  443057

• UniProt :

  P19397

• Swiss Prot :

  P19397

• Host :

  Mouse

• Species Reactivity :

  Human

• Reactivity :

  CD53

• Immunogen :

  Human Sezary cells

• Target Antigen :

  CD53

• Clonality :

  Monoclonal

• Clone :

  63-5A3

• Type :

  Primary

• Source :

  Animal

• Origin :

  Animal

• Applications :

  Flow, surface|Functional studies (published) |IP (published)

• Field of Research :

  Immunology

• Positive Control :

  Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

• Stability :

  2 years

• Concentration :

  0.2 mg/mL

• Buffer :

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight :

  33-55 kDa

• References & Citations :

  Note: References for this clone sold by other suppliers may be listed for expected applications. Eur J Biochem (2002) 269, 1012-1021. (functional studies) J Immunol (1990) 145:4322-4325. (IP) Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989. Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997.

• Shipping Conditions :

  RT (ND)

• Storage Conditions :

  Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life :

  Guaranteed for at least 24 months from date of receipt when stored as recommended

• Fragment :

  IgG2b, kappa

• Species :

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification :

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number :

  9007-83-4

• Conjugate :

  Purified, with BSA