CD53 (63-5A3), 0.2mg/mL

• Description:

  Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop V; Code CD53.1) . CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD53

• Gene ID:

  963

• NCBI Gene ID:

  443057

• UniProt:

  P19397

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human Sezary cells

• Target Antigen:

  CD53

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  63-5A3

• Conjugation:

  Purified, with BSA

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  Flow, surface|Functional studies (published) |IP (published)

• Validated Applications:

  FC, IP

• Field of Research:

  Immunology

• Positive Control:

  Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  33-55 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Eur J Biochem (2002) 269, 1012-1021. (functional studies) J Immunol (1990) 145:4322-4325. (IP) Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989. Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997.

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA