CD10 (FR4D11), CF488A conjugate, 0.1mg/mL

• Description:

  CD10 (also known as Common Acute Lymphocytic Leukemia Antigen (CALLA) ) is a type II transmembrane protein. It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML) . It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD10; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen (CALLA); Enkephalinase; gp100; Membrane metalloendopeptidase; Neutral endopeptidase

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  MME

• Gene ID:

  4311

• NCBI Gene ID:

  307734

• UniProt:

  P08473

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Raji cells

• Target Antigen:

  CD10

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  FR4D11

• Conjugation:

  CF488A

• Disease:

  Tumor

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  Flow (verified) |Flow, surface (published) |IF (published) |IF (verified)

• Validated Applications:

  FC, FC, IF, IF

• Field of Research:

  Immunology, Proteases

• Positive Control:

  Tonsil, small intestine, kidney

• Stability:

  2 years

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  100 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Oncotarget (2015) 6(4): 2315-2330. (Flow, surface) Oncotarget (2016) 7(34): 54157-54173. (IF)

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Notes:

  Protect from light

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  CF488A