Recombinant Mycobacterium tuberculosis Transporter PPE51 (PPE51)

Recombinant Mycobacterium tuberculosis Transporter PPE51 (PPE51)

• CAS Number: 9000-83-3
• Gene Name: PPE51
• UniProt: P9WHY2
• Expression Region: 1-380aa
• Organism: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
• Target Sequence: MDFALLPPEVNSARMYTGPGAGSLLAAAGGWDSLAAELATTAEAYGSVLSGLAALHWRGPAAESMAVTAAPYIGWLYTTAEKTQQTAIQARAAALAFEQAYAMTLPPPVVAANRIQLLALIATNFFGQNTAAIAATEAQYAEMWAQDAAAMYGYATASAAAALLTPFSPPRQTTNPAGLTAQAAAVSQATDPLSLLIETVTQALQALTIPSFIPEDFTFLDAIFAGYATVGVTQDVESFVAGTIGAESNLGLLNVGDENPAEVTPGDFGIGELVSATSPGGGVSASGAGGAASVGNTVLASVGRANSIGQLSVPPSWAAPSTRPVSALSPAGLTTLPGTDVAEHGMPGVPGVPVAAGRASGVLPRYGVRLTVMAHPPAAG
• Tag: N-terminal 6xHis-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: Developed Protein
• Relevance: Small molecule-selective channel required for the uptake of nutrients across the outer mycomembrane. Transports glycerol and glucose. Involved in sensitivity to M.tuberculosis growth inhibitory agrichemical 3, 3-bis-di (methylsulfonyl)propionamide (3bMP1). Transports maltose and lactose disaccharides. Involved in sensitivity to bactericidal thio-disaccharide T-6 compound (1, 6-anhydro-3-deoxy-4-S- (2, 3, 4, 6-tetra-O-acetyl-beta-D-glucopyranosyl)-D-glycero-hexopyranos-2-ulose). Transports extracellular trehalose, a component of the cell envelope, and trehalose analog, 6-azido trehalose (6-TreAz), which has antimycobacterial activity.
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Bioactivity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 44.0 kDa
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.