Rat PIIINP (Procollagen III N-Terminal Propeptide) ELISA Kit

Rat PIIINP (Procollagen III N-Terminal Propeptide) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
• Field of Research: Metabolic pathway; Hepatology; Bone metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 400 ng/mL
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 6.25-400 ng/mL
• Sensitivity: 2.13 ng/mL