Human Transferrin Protein, His tag

• Background:

  Transferrin (Tf), is an iron transporter, which is mainly biosynthesized in the liver, and it can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc.The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. Biochemical analyses reveal that the apparent molecular size of brain-type Tf is smaller than that of serum-type Tf, and that hydrophobic patches are exposed on brain-type Tf as demonstrated by hydrophobic probe binding studies. Research indicates that brain-type Tf levels is decreased in idiopathic normal pressure hydrocephalus, in which CSF production is suspected to decrease, while brain-type Tf increased in spontaneous intracranial hypotension, in which CSF production is suspected to increase. These results suggest that brain-type Tf could be a biomarker of altered CSF production.

• Description:

  Human Transferrin protein, expressed in HEK293 Cells

• Product Name Alternative:

  PRO2086 Protein; TFQTL1 Protein; HEL-S-71p Protein, Human; PRO1557 Protein; Transferrin Protein, Siderophilin, partially saturated

• Gene ID:

  7018

• Accession Number:

  P02787

• Tag:

  TF

• Type:

  Cytokines and proteins

• Applications:

  His tag

• Purity:

  ≥ 95 % as determined by SDS-PAGE

• Activity:

  Testing in progress

• Molecular Weight:

  76.6 kDa

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Features & Benefits:

  Endotoxin: < 1 EU per µg of the protein as determined by the LAL method

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Lyophilized Human Transferrin protein should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Protein Length:

  The recombinant Human Transferrin protein consists of 690 amino acids and has a predicted molecular mass of 76.6 kDa. The apparent molecular mass of the Human Transferrin protein is approximately 70-100 kDa in SDS-PAGE under reducing conditions.

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.

• Applications Notes:

  Human

• Other Product Names:

  PRO2086 Protein; TFQTL1 Protein; HEL-S-71p Protein, Human; PRO1558 Protein; Transferrin Protein, Siderophilin, partially saturated

• Recommended Usage:

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Transferrin protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

• Preparation:

  HEK293 Cells

• Accession Number URL:

  https://www.uniprot.org/uniprot/P02787

• AA Sequence:

  Amino acid sequence derived from Human Transferrin protein isoform (P02787, Met 1-Pro 698) with a polyhistidine tag at the C-terminus was expressed.