Mouse anti-COX4-1 Antibody

• Background:

  Mouse monoclonal antibody to COX4-1

• Synonyms:

  COX4; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1

• Host:

  Mouse

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant protein corresponding to human COX4-1.

• Clonality:

  Monoclonal

• Applications:

  WB, IF/IC

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of COX4-1 expression in Hela (A), HepG2 (B), 293T (C), mouse heart (D), mouse muscle (E), rat heart (F) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of COX4-1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

• Gene ID (Human):

  1327

• Gene ID (Mouse):

  12857

• Gene ID (Rat):

  29445

• SwissProt (Human):

  P13073

• SwissProt (Mouse):

  P19783

• SwissProt (Rat):

  P10888

• CAS Number:

  9007-83-4