Rabbit anti-SAP14 Antibody

• Background:

  Rabbit polyclonal antibody to SAP14

• Synonyms:

  SAP14; SF3B14; SF3B14A; Splicing factor 3B subunit 6; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6,14kDa

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, Z

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SAP14. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of SAP14 expression in HepG2 (A), RT4 (B) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  51639

• Gene ID (Mouse):

  66055

• SwissProt (Human):

  Q9Y3B4

• SwissProt (Mouse):

  P59708

• CAS Number:

  9007-83-4