Rabbit anti-p38 (pT180) Antibody

• Background:

  Rabbit polyclonal antibody to p38 (pT180)

• Synonyms:

  CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a

• Host:

  Rabbit

• Reactivity:

  H, M, R, D, Z

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p38 (pT180) . The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC, IP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of p38 (pT180) expression in HepG2 PMA-treated (A), NIH3T3 (B), PC12 (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of p38 (pT180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  1432

• Gene ID (Mouse):

  26416

• SwissProt (Human):

  Q16539

• SwissProt (Mouse):

  P47811

• SwissProt (Rat):

  P70618

• CAS Number:

  9007-83-4