Rabbit anti-HSC70 Antibody

• Background:

  Rabbit polyclonal antibody to HSC70

• Synonyms:

  HSC70; HSP73; HSPA10; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, C, Mk, P

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC, IP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of HSC70 expression in HeLa (A), NIH3T3 (B) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of HSC70 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  3312

• Gene ID (Mouse):

  15481

• Gene ID (Rat):

  24468

• SwissProt (Human):

  P11142

• SwissProt (Mouse):

  P63017

• SwissProt (Rat):

  P63018

• CAS Number:

  9007-83-4