Rabbit anti-Gamma-tubulin Antibody

• Background:

  Rabbit polyclonal antibody to Gamma-tubulin

• Synonyms:

  TUBG; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1

• Host:

  Rabbit

• Reactivity:

  H, Mk

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-tubulin. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Gamma-tubulin expression in A549 (A), HeLa (B), HEK293T (C), NIH3T3 (D) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Gamma-tubulin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  7283

• SwissProt (Human):

  P23258

• CAS Number:

  9007-83-4