Rabbit anti-FOXD3 Antibody

• Background:

  Rabbit polyclonal antibody to FOXD3

• Synonyms:

  HFH2; Forkhead box protein D3; HNF3/FH transcription factor genesis

• Host:

  Rabbit

• Reactivity:

  H, M, R, C

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXD3. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Rabbit

• WB Description:

  Western blot analysis of FOXD3 expression in Jurkat (A), K562 (B), NIH3T3 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of FOXD3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  27022

• Gene ID (Mouse):

  15221

• SwissProt (Human):

  Q9UJU5

• SwissProt (Mouse):

  Q61060

• SwissProt (Rat):

  Q63245

• CAS Number:

  9007-83-4