Rabbit anti-Cytochrome P450 11A1 Antibody

• Background:

  Rabbit polyclonal antibody to Cytochrome P450 11A1

• Synonyms:

  CYP11A; Cholesterol side-chain cleavage enzyme, mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450 (scc)

• Host:

  Rabbit

• Reactivity:

  H, M, R, Mk

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytochrome P450 11A1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Cytochrome P450 11A1 expression in Jurkat (A), mouse liver (B), rat liver (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of Cytochrome P450 11A1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

• Gene ID (Human):

  1583

• Gene ID (Mouse):

  13070

• Gene ID (Rat):

  29680

• SwissProt (Human):

  P05108

• SwissProt (Mouse):

  Q9QZ82

• SwissProt (Rat):

  P14137

• CAS Number:

  9007-83-4