Rabbit anti-Carbonic Anhydrase 2 Antibody

• Background:

  Rabbit polyclonal antibody to Carbonic Anhydrase 2

• Synonyms:

  Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II

• Host:

  Rabbit

• Reactivity:

  H, R, Mk

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Carbonic Anhydrase 2. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Carbonic Anhydrase 2 expression in Caco2 (A), K562 (B), Caki1 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Carbonic Anhydrase 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  760

• Gene ID (Rat):

  54231

• SwissProt (Human):

  P00918

• SwissProt (Rat):

  P27139

• CAS Number:

  9007-83-4