Prohibitin (Mitochondrial Marker) (PHB/3229), 0.2mg/mL

• Description:

  Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB1; Prohibitin

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PHB

• Gene ID:

  5245

• NCBI Gene ID:

  514303

• UniProt:

  P35232

• Cellular Locus:

  Mitochondria

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)

• Target Antigen:

  Prohibitin

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PHB/3229

• Conjugation:

  Purified, with BSA

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Positive Control:

  Liver, Kidney, Heart or Endometrium (IHC).

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  ~30 kDa

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA