Recombinant Human RSPO1

• Product Name Alternative:

  RSPO1, R-spondin1, RP11-566C13.1, CRISTIN3, FLJ40906, RSPO Rspo1, R-spondin, Rspondin, RP23-325M14.2, Roof plate-specific spondin-1

• UniProt:

  Q2MKA7

• Expression Region:

  21-263

• Host:

  Mammalian Cells

• Tag:

  C-terminal His Tag

• Applications:

  Western Blot, ELISA

• Endotoxin:

  < 0.01 EU/µg as determined by LAL test.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10ng/ml.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  40 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

• Organism Species:

  Human