Rabbit anti-JNK3 Antibody

• Background:

  Rabbit polyclonal antibody to JNK3

• Synonyms:

  JNK3; JNK3A; PRKM10; SAPK1B; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JNK3. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC, IP

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of JNK3 expression in NIH3T3 (A), SKNSH (B), HeLa (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of JNK3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  5602

• Gene ID (Mouse):

  26414

• Gene ID (Rat):

  25272

• SwissProt (Human):

  P53779

• SwissProt (Mouse):

  Q61831

• SwissProt (Rat):

  P49187

• CAS Number:

  9007-83-4