Rabbit anti-MCCB Antibody

• Background:

  Rabbit polyclonal antibody to MCCB

• Synonyms:

  MCCB; Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial; MCCase subunit beta; 3-methylcrotonyl-CoA carboxylase 2; 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta

• Host:

  Rabbit

• Reactivity:

  H

• Immunogen:

  Recombinant full length protein of human MCCB

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of MCCB expression in Hela (A), HepG2 (B), mouse liver (C), mouse heart (D), rat brain (E) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of MCCB staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  64087

• SwissProt (Human):

  Q9HCC0

• CAS Number:

  9007-83-4