Recombinant Escherichia coli Chaperone protein fimC (fimC)

Recombinant Escherichia coli Chaperone protein fimC (fimC)

• CAS Number: 9000-83-3
• Gene Name: fimC
• UniProt: P31697
• Expression Region: 37-241aa
• Organism: Escherichia coli (strain K12)
• Target Sequence: GVALGATRVIYPAGQKQEQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALTPKMTGVME
• Tag: N-terminal GST-tagged
• Source: E.coli
• Field of Research: Microbiology
• Assay Type: Developed Protein
• Relevance: Required for the biogenesis of type 1 fimbriae. Binds and interact with FimH.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length of Mature Protein
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 50.2 kDa
• References & Citations: "FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria." Jones C.H., Pinkner J.S., Nicholes A.V., Slonim L.N., Abraham S.N., Hultgren S.J. Proc. Natl. Acad. Sci. U.S.A. 90:8397-8401 (1993)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.