Estrogen Receptor Antibody

• Description:

  This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

• Specifications:

  Western blot: 1-2 µg/mL for 2 hours at RT

• UniProt:

  P03372

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human Estrogen Receptor alpha protein (amino acids 2-185) was used as the immunogen for this Estrogen Receptor alpha antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM567

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Estrogen Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Estrogen Receptor antibody is available for research use only.

• Storage Conditions:

  Store the Estrogen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The optimal dilution of the Estrogen Receptor antibody for each application should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor antibody (SPM567) .