GMCSF Antibody

• Description:

  Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P04141

• Host:

  Rat

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for this GMCSF antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CSF2/3403

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the GMCSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This GMCSF antibody is available for research use only.

• Storage Conditions:

  Store the GMCSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GM-CSF antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Secreted (extracellular)

• Image Legend:

  IHC staining of FFPE human spleen with GMCSF antibody (clone CSF2/3403) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.