Recombinant MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase

• Description:

  Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase) . It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

• Specifications:

  ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Q13126

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  MTAP/3137R

• Type:

  Recombinant

• Applications:

  ELISA, WB, IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant MTAP antibody is available for research use only.

• Storage Conditions:

  Store the recombinant MTAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic

• Image Legend:

  IHC staining of FFPE human kidney with recombinant MTAP antibody. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.