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Ki-67 Antibody

CAT: 0800-V7432-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V7432-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ki67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki67 index and the histo-pathological grade of neoplasms. Assessment of Ki67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki67 is a good marker of proliferating cell populations.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P46013
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 2293-2478 was used as the immunogen for the Ki-67 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
MKI67/2466
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Ki-67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Ki-67 antibody is available for research use only.
Storage Conditions
Store the Ki-67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
Immunofluorescent staining of human HeLa cells with Ki-67 antibody (green, clone MKI67/2466) and Phalloidin (purple, membrane stain) .

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