EGF Receptor Antibody / Epidermal Growth Factor Receptor

• Description:

  This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

• Specifications:

  Western blot: 1-2 µg/mL

• UniProt:

  P00533

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 985-996 from the human protein were used as the immunogen for the EGF Receptor antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  F4

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the EGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This EGF Receptor antibody is available for research use only.

• Storage Conditions:

  Store the EGF Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titering of the EGF Receptor antibody may be required for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  Western blot testing of human A431 cell lysate with EGF Receptor antibody (clone F4) . Expected molecular weight: 134-180 kDa depending on glycosylation level.