Recombinant Cytokeratin 18 Antibody

• Description:

  This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P05783

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Full length recombinant human protein was used as the immunogen for the recombinant Cytokeratin 18 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  KRT18/2819R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the recombinant Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant Cytokeratin 18 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic

• Image Legend:

  IHC staining of FFPE human colon carinoma with recombinant Cytokeratin 18 antibody (clone KRT18/2819R) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.