Recombinant CD20 Antibody / Rabbit Monoclonal

• Description:

  CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P11836

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  IGEL/1497R

• Type:

  Recombinant

• Applications:

  FACS, IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This recombinant CD20 antibody is available for research use only.

• Storage Conditions:

  Recombinant CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Titering of the recombinant CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9000-83-3

• Location:

  Predominantly cell surface with some cytoplasmic

• Image Legend:

  IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.