Recombinant PD-1 Antibody / Rabbit Monoclonal

• Description:

  Programmed cell death protein 1 is an inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors. [UniProt]

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  Q15116

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A full length recombinant human protein was used as the immunogen for the recombinant PD-1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  RMPD1-1

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the recombinant PD-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant PD-1 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant PD-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titering of the recombinant PD-1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9000-83-3

• Location:

  Cell surface & cytoplasmic

• Image Legend:

  IHC testing of FFPE human tonsil with recombinant PD-1 antibody (clone RMPD1-1) . HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.