Anti-CD34 Antibody

• Description:

  This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens) . Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

• UniProt:

  P28906

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the anti-CD34 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  HPCA1/1171

• Applications:

  FACS, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the anti-CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This anti-CD34 antibody is available for research use only.

• Storage Conditions:

  Store the anti-CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD34 antibody (HPCA1/1171)