Anti-CD19 Antibody

• Description:

  CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. It is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P15391

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human CD19 recombinant protein was used as immunogen for this anti-CD19 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CVID3/155

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Anti-CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This anti-CD19 antibody is available for research use only.

• Storage Conditions:

  Anti-CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD19 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Flow cytometry testing of lymphocyte-gated human PBM cells with anti-CD19 antibody (clone CVID3/155) ; Gray=unstained cells, Orange= anti-CD19 antibody stained cells.