SEMA3B Antibody / Semaphorin 3B

• Description:

  Semaphorin-3B is a protein that in humans is encoded by the SEMA3B gene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.

• Specifications:

  Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  Q13214

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human protein (amino acids Y55-R624) was used as the immunogen for the SEMA3B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  9C4F7

• Applications:

  WB, IF, FACS

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the SEMA3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This SEMA3B antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the SEMA3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the SEMA3B antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human HeLa cells with SEMA3B antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.