Erlin-2 Antibody

• Description:

  Erlin-2 is a protein that in humans is encoded by the ERLIN2 gene. This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18) . Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

• Specifications:

  Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  O94905

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An E. coli-derived human protein (amino acids D87-N339) was used as the immunogen for the Erlin-2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, FACS, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Erlin-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Erlin-2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Erlin-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Erlin-2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human T-47D cells with Erlin-2 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.