BRM Antibody / SMARCA2

• Description:

  Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. It is mapped to 9p24.3. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P51531

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein (amino acids Q181-N624) was used as the immunogen for the BRM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  3G3

• Applications:

  WB, IF, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the BRM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This BRM antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the BRM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the BRM antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of human A431 cells with BRM antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= BRM antibody.