Nix Antibody / BNIP3L

• Description:

  'BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like' (BNIP3L), also called NIP3-like protein X (Nix) induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. [UniProt]

• Specifications:

  Western blot: 1:500-1:2000

• UniProt:

  O60238

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human Nix / BNIP3L was used as the immunogen for the Nix antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  ABIB-2

• Type:

  Recombinant

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the Nix antibody at -20oC.

• Limitations:

  This Nix antibody is available for research use only.

• Storage Conditions:

  Store the Nix antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the Nix antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot testing of human K562 cell lysate with Nix antibody. Expected molecular weight: ~38 kDa (monomer), ~76 kDa (homodimer) .