Recombinant Histone H2A Antibody

• Description:

  This recombinant Histone H2A antibody reacts to the Histone H2A protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

• Specifications:

  Western blot: 0.5-2 µg/mL, Immunocytochemistry: 1-2 µg/mL, ELISA: 0.2-1 µg/mL

• UniProt:

  P04908, P0C0S8, P20671

• Host:

  Rabbit

• Reactivity:

  All Species Expected

• Immunogen:

  A peptide corresponding to the C-terminus of human Histone H2A was used as the immunogen for this recombinant Histone H2A antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RM225

• Type:

  Recombinant

• Applications:

  WB, ELISA, ICC

• Purity:

  Protein A purified from animal origin-free supernatant

• Format:

  Purified

• Buffer:

  1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution:

  Store the recombinant Histone H2A antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

• Limitations:

  This recombinant Histone H2A antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Histone H2A antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

• Formulation:

  1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the recombinant Histone H2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot of recombinant Histone H2A protein, H2B protein and whole cell lysates of human HeLa, A375, SK-MEL-2, A431, and K562 cells using recombinant Histone H2A antibody at 0.5 ug/ml. Predicted molecular weight ~14 kDa.