Recombinant H3K79me1 Antibody

• Description:

  This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1) . No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.

• Specifications:

  Western blot: 0.2-1 µg/mL, ChIP: 2-10ug/mg of lysate, ELISA: 0.2-1 µg/mL

• UniProt:

  P84243

• Host:

  Rabbit

• Reactivity:

  All Species Expected

• Immunogen:

  A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RM147

• Type:

  Recombinant

• Applications:

  WB, ELISA, ChIP

• Purity:

  Protein A purified from animal origin-free supernatant

• Format:

  Purified

• Buffer:

  1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution:

  Store the recombinant H3K79me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

• Limitations:

  This recombinant H3K79me1 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant H3K79me1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

• Formulation:

  1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me1 antibody at 0.5 ug/ml showed a band of Histone H3 monomethylated at Lysine 79 (K79me1) in HeLa cells.