Pyruvate dehydrogenase kinase 2 Antibody / PDK2

• Description:

  Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt:

  Q9JK42

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 78-107 from the mouse protein was used as the immunogen for the Pyruvate dehydrogenase kinase 2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Pyruvate dehydrogenase kinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Pyruvate dehydrogenase kinase 2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Pyruvate dehydrogenase kinase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Pyruvate dehydrogenase kinase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE mouse heart tissue with Pyruvate dehydrogenase kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.