Cystathionine Beta Synthase Antibody / CBS

• Description:

  CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.

• Specifications:

  Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

• UniProt:

  P35520

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 104-133 from the human protein was used as the immunogen for the Cystathionine Beta Synthase antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, WB, FACS, IHC-P

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Cystathionine Beta Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Cystathionine Beta Synthase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Cystathionine Beta Synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Cystathionine Beta Synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  Western blot testing of 1) mouse kidney and 2) human Ramos lysate with Cystathionine Beta Synthase antibody. Predicted molecular weight ~61 kDa.