PKN1 Antibody

• Description:

  PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR) -dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

• Specifications:

  Western blot: 1:2000

• UniProt:

  Q16512

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids 836-870 from the human protein were used as the immunogen for this PKN1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Limitations:

  This PKN1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the PKN1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the PKN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Bovine

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of PKN1 antibody at 1:2000 dilution + human Jurkat whole cell lysate. Predicted molecular weight ~104 kDa.