C5 Antibody

• Description:

  C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.

• Specifications:

  Western blot: 1:1000, Immunohistochemistry (Paraffin) : 1:50-1:100, Flow cytometry: 1:10-1:50

• UniProt:

  P01031

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 253-281 from the human protein was used as the immunogen for this C5 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, FACS, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the C5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This C5 antibody is available for research use only.

• Storage Conditions:

  Aliquot the C5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the C5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of C5 antibody and HL-60 lysate