Alexa Fluor™ 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (Monomer)

Alexa Fluor™ 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (Monomer)

• Background: The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*1101 KRASG12D (VVVGADGVGK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and VVVGADGVGK peptide of the KRASG12D.
• Description: Alexa Fluor 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (HLD-HA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGADGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGADGVGK).
• Specifications: Alexa Fluor 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12D peptide. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 49.4 kDa and 11.7 kDa. The protein migrates as 57-70 kDa and 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGADGVGK peptide
• Host: HEK 293
• Target: HLA-A*1101 & B2M & KRASG12D (VVVGADGVGK)
• Conjugation: AF488 Labeled
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Alexa Fluor 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (Cat. No. HLD-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 49.4 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light