Rabbit anti Human SURF1

Rabbit anti Human SURF1

• Background: Human Ssu72 is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/termination machinery. The Human Ssu72 is located on Chromosome 1p36 and seems to represent a single gene. It was identified as a pRb binding factor and was also shown to interact with TFIIB and yeast PTA1. Rabbit polyclonal antibodies were raised against SSU72 fusion protein. The ABs recognize a single band of ~30 Kda on immunoblots of various Human cell lines but do not work well on mouse tissues.
• Synonyms: Human Ssu72
• UniProt: Q15526
• Host: Rabbit
• Species Reactivity: Human
• Isotype: IgG
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Applications: WB
• Field of Research: Signal Transduction
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: Antibody can be used for Western blot (1-5 µg/mL). Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. St-Pierre, B., et al. 'Conserved and specific functions of mammalian ssu72' Nucleic Acids Res. 2005, 33, 464-477 2. Krishnamurthy, S., et al. 'Ssu72 is an RNA polymerase II CTD phosphatase' Mol. Cell 2004, 14, 387-394 3. Steinmetz, EJ., Brow, DA. 'Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription' Mol Cell Biol 2003, 23, 6339-6349
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB